Use of Open Pulled Straw as a Carrier in Vitrification of Metaphase II Oocyte in Mice
نویسندگان
چکیده
منابع مشابه
P-102: Effect of Vitrification in Open Pulled Straw Vitrification of Live Birth Rates, Fertility and The Development of Mouse Oocytes at Metaphase II
Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...
متن کاملEffect of vitrification on number of inner cell mass in mouse blastocysts in conventional straw, closed pulled straw, open pulled straw and cryoloop carriers.
OBJECTIVE To compare the effect of using open and closed carriers on count of inner cell mass in vitrified mouse blastocyst after warming. METHODS The experimental study was conducted at Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, from April to September 2010. Forty female NMRI (Naval Medical Research Institute, USA) mice were injected with pregnant mare's serum gonadotropi...
متن کاملThe Survival Rate, Fertilization and Developmental Capacity of Mature Mouse Oocyte Which Vitrified in Open Pulled Straw
Purpose: The aim of this study was to evaluate the effect of vitrification in open pulled straw on survival rate, fertilization and development capacity and comparison with conventional straw. Materials and Methods: NMRI mice were used for retrieval of oocytes. After administration of HMG and HCG, the mice scarified by cervical dislocation. Mice's abdomens were dissected and mature oocytes wer...
متن کاملComparison of The Effects of Vitrification on Gene Expression of Mature Mouse Oocytes Using Cryotop and Open Pulled Straw
Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...
متن کاملVitrification of immature bubaline cumulus oocyte complexes by the open-pulled straw and conventional straw methods and their subsequent in vitro fertilization
The in vitro maturation (IVM), fertilization (IVF) and morphological changes in buffalo cumulus oocyte complexes (COCs) cryopreserved by ultra rapid freezing using conventional (CON) and open-pulled straw (OPS) methods were tested. COCs were cryopreserved using a vitrification solution comprising of DPBS + 0.5M sucrose + 0.4% BSA and two concentrations (4.5 or 5.5M) of each cryoprotectant ethyl...
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ژورنال
عنوان ژورنال: Asian Journal of Biological Sciences
سال: 2010
ISSN: 1996-3351
DOI: 10.3923/ajbs.2010.85.91